Production of rebaudioside D from stevioside using a UGTSL2 Asn358Phe mutant in a multi‐enzyme system
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چکیده
منابع مشابه
Synthesis of rebaudioside A from stevioside and their interaction model with hTAS2R4 bitter taste receptor.
Steviol glycosides (SG's) from Stevia rebaudiana (Bertoni) have been used as a natural low-calorie sweeteners. Its aftertaste bitterness restricts its use for human consumption and limits its application in food and pharmaceutical products. In present study, we have performed computational analysis in order to investigate the interaction of two major constituents of SG's against homology model ...
متن کاملBioconversion of rebaudioside I from rebaudioside A.
To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT,...
متن کاملA Rapid Dialysis Method for Analysis of Stevioside and Rebaudioside A in Foods.
A rapid dialysis method for the analysis of stevioside (SS) and rebaudioside A (RS) in foods was developed. Minced samples (10 g) were packed into 30 cm net length dialysis tubing with 30% methanol to increase the dialysis efficiency. The dialysis tubing was put in a 100 mL centrifuge tube, and the total fluid volume was made up to 100 mL with 30% methanol. Dialysis was done with shaking while ...
متن کاملEffect of salicylic acid on stevioside and rebaudioside A production and transcription of biosynthetic genes in in vitro culture of Stevia rebaudiana
S. rebaudiana produces steviol glycosides including stevioside and rebaudioside A that are valuable as low calorie sweeteners. The objective of this study was to investigate the effects of salicylic acid elicitation and sampling times on the improvment of stevioside and rebaudioside A production and KA13H, UGT74G1 and UGT76G1 genes expression. The results showed that the addition of different c...
متن کاملProduction of b-Fructofuranosidase by Arthrobacter sp. and Its Application in the Modification of Stevioside and Rebaudioside A
Arthrobacter sp. 10137 has been used to produce b-fructofuranosidase (FFase). Sucrose and corn steep powder in an optimized ratio of 10:1 were the best carbon and nitrogen sources for enzyme production in a shake flask. The maximum FFase activity was 26.69 U/mL after 22.5 h in batch culture, and the crude FFase, obtained by ultrafiltration and (NH4)2SO4 fractionation, was purified about 7-fold ...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2020
ISSN: 1751-7915,1751-7915
DOI: 10.1111/1751-7915.13539